Instrument was shot from 1987 through 1998 on super 8, 16mm and video and is composed mainly of footage of concerts, interviews with the band members, practices, tours and time spent in the studio recording their 1995 album, Red Medicine. The film also includes portraits of fans as well as interviews with them at various Fugazi shows around the United States throughout the years.

Notable scenes in the film include Fugazi performing for inmates at Lorton Correctional Facility; singer/guitarist Guy Picciotto's performance in a Philadelphia college gym in 1988, where he stuffs himself through a basketball hoop and performs hanging upside down by his legs (filmed by Todd Crespi); and an interview of Guy Picciotto and Ian MacKaye by an 8th grade girl for a Public-access television cable TV show.